Prophylactic, therapeutic and immune enhancement effect of liposome‐encapsulated PolyICLC on highly pathogenic H5N1 influenza infection
Identifieur interne : 001420 ( Main/Exploration ); précédent : 001419; suivant : 001421Prophylactic, therapeutic and immune enhancement effect of liposome‐encapsulated PolyICLC on highly pathogenic H5N1 influenza infection
Auteurs : Yi Li [République populaire de Chine] ; Yanxin Hu [République populaire de Chine] ; Yi Jin [République populaire de Chine] ; Guozhong Zhang [République populaire de Chine] ; Jonathan Wong [Canada] ; Lun-Quan Sun [République populaire de Chine, Australie] ; Ming Wang [République populaire de Chine]Source :
- The Journal of Gene Medicine [ 1099-498X ] ; 2011-01.
English descriptors
- Teeft :
- Adjuvant, Alveolar spaces, Alveolar walls, Antibody titres, Antigen pool, Assay, Avian viruses, Body weight, Body weights, Bovine serum albumin, Brosis, Cellular responses, Control group, Control mice, Copyright, Cytokine, Days data, Enhancement, Higher level, Higher percentages, Histopathological changes, Hyclone laboratories, Igg2a, Immune, Immune enhancement, Immune enhancement agent, Immune enhancement effect, Immune response, Immune responses, Immunization, Immunol, Infection, Interstitial oedema, Intranasal administration, John wiley sons, Lepolyiclc, Liposomeencapsulated polyiclc, Lung histopathology, Lung structures, Lung tissue, Lung tissues, Lysis, Mdck cells, Molecular adjuvant, Molecular medicine, Monoclonal antibody, Mouse, Mucous epithelium, Negative control, Pathogenic, Pathogenic viruses, Plaque assay, Poly, Poly iclc, Polyiclc, Positive control, Present study, Primer, Proliferative response, Prophylactic, Prophylactic effect, Protective immunity, Public health, Replication, Representative lung sections, Respir crit care, Respiratory failure, Restoration period, Same doses, Same time, Second immunization, Serum samples, Small blood vessels, Stimulation index, Survival rate, Target cells, Therapeutic agent, Therapeutic agents, Therapeutic effects, Titre, Total survival, Triplicate wells, Vaccination, Vaccine, Veterinary medicine, Viral, Viral challenge, Viral clearance, Viral infection, Viral infections, Viral load, Viral replication, Viral titres, Virus, Virus infection, Virus replication, Xiangya hospital.
Abstract
Background: In view of the magnitude and severity of outbreaks of the highly pathogenic H5N1 influenza virus (H5N1‐HPIV) and the threat to public health, there is an urgent need to develop broad‐spectrum prophylactic and therapeutic agents against infection by H5N1‐HPIV and other subtypes. Methods and Results: In the present study, we explored the use of LE‐PolyICLC, a liposome encapsulated double‐stranded RNA, as a possible prophylactic, therapeutic and immune enhancement agent. In a mouse infection model, we showed that the administration of LE‐PolyICLC intranasally before or shortly after infection could inhibit virus replication, leading to a significant reduction in pulmonary viral titres and a higher survival rate of infected mice. When used as a molecular adjuvant, LE‐PolyICLC significantly enhanced both the humoral and cellular responses elicited by inactivated H5N1 vaccine and augmented the protective efficacy provided by vaccination. Most importantly, the data also demonstrate that LE‐PolyICLC could effectively attenuate the development of pulmonary fibrosis during the restoration period at day 14 after H5N1 infection. Conclusions: Taken together, the data obtained in the present study suggest that strong consideration should be given for the use of LE‐PolyICLC as prophylactic and therapeutic agents and also as a vaccination adjuvant to combat highly pathogenic influenza infection and its associated complications such as pulmonary fibrosis. Copyright © 2011 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/jgm.1536
Affiliations:
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to="72">72</biblScope><biblScope unit="page-count">13</biblScope><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2011-01">2011-01</date></imprint><idno type="ISSN">1099-498X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1099-498X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adjuvant</term><term>Alveolar spaces</term><term>Alveolar walls</term><term>Antibody titres</term><term>Antigen pool</term><term>Assay</term><term>Avian viruses</term><term>Body weight</term><term>Body weights</term><term>Bovine serum albumin</term><term>Brosis</term><term>Cellular responses</term><term>Control group</term><term>Control mice</term><term>Copyright</term><term>Cytokine</term><term>Days data</term><term>Enhancement</term><term>Higher level</term><term>Higher percentages</term><term>Histopathological changes</term><term>Hyclone laboratories</term><term>Igg2a</term><term>Immune</term><term>Immune enhancement</term><term>Immune enhancement agent</term><term>Immune enhancement effect</term><term>Immune response</term><term>Immune responses</term><term>Immunization</term><term>Immunol</term><term>Infection</term><term>Interstitial oedema</term><term>Intranasal administration</term><term>John wiley sons</term><term>Lepolyiclc</term><term>Liposomeencapsulated polyiclc</term><term>Lung histopathology</term><term>Lung structures</term><term>Lung tissue</term><term>Lung tissues</term><term>Lysis</term><term>Mdck cells</term><term>Molecular adjuvant</term><term>Molecular medicine</term><term>Monoclonal antibody</term><term>Mouse</term><term>Mucous epithelium</term><term>Negative control</term><term>Pathogenic</term><term>Pathogenic viruses</term><term>Plaque assay</term><term>Poly</term><term>Poly iclc</term><term>Polyiclc</term><term>Positive control</term><term>Present study</term><term>Primer</term><term>Proliferative response</term><term>Prophylactic</term><term>Prophylactic effect</term><term>Protective immunity</term><term>Public health</term><term>Replication</term><term>Representative lung sections</term><term>Respir crit care</term><term>Respiratory failure</term><term>Restoration period</term><term>Same doses</term><term>Same time</term><term>Second immunization</term><term>Serum samples</term><term>Small blood vessels</term><term>Stimulation index</term><term>Survival rate</term><term>Target cells</term><term>Therapeutic agent</term><term>Therapeutic agents</term><term>Therapeutic effects</term><term>Titre</term><term>Total survival</term><term>Triplicate wells</term><term>Vaccination</term><term>Vaccine</term><term>Veterinary medicine</term><term>Viral</term><term>Viral challenge</term><term>Viral clearance</term><term>Viral infection</term><term>Viral infections</term><term>Viral load</term><term>Viral replication</term><term>Viral titres</term><term>Virus</term><term>Virus infection</term><term>Virus replication</term><term>Xiangya hospital</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: In view of the magnitude and severity of outbreaks of the highly pathogenic H5N1 influenza virus (H5N1‐HPIV) and the threat to public health, there is an urgent need to develop broad‐spectrum prophylactic and therapeutic agents against infection by H5N1‐HPIV and other subtypes. Methods and Results: In the present study, we explored the use of LE‐PolyICLC, a liposome encapsulated double‐stranded RNA, as a possible prophylactic, therapeutic and immune enhancement agent. In a mouse infection model, we showed that the administration of LE‐PolyICLC intranasally before or shortly after infection could inhibit virus replication, leading to a significant reduction in pulmonary viral titres and a higher survival rate of infected mice. When used as a molecular adjuvant, LE‐PolyICLC significantly enhanced both the humoral and cellular responses elicited by inactivated H5N1 vaccine and augmented the protective efficacy provided by vaccination. Most importantly, the data also demonstrate that LE‐PolyICLC could effectively attenuate the development of pulmonary fibrosis during the restoration period at day 14 after H5N1 infection. Conclusions: Taken together, the data obtained in the present study suggest that strong consideration should be given for the use of LE‐PolyICLC as prophylactic and therapeutic agents and also as a vaccination adjuvant to combat highly pathogenic influenza infection and its associated complications such as pulmonary fibrosis. Copyright © 2011 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Canada</li><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Li, Yi" sort="Li, Yi" uniqKey="Li Y" first="Yi" last="Li">Yi Li</name></noRegion><name sortKey="Hu, Yanxin" sort="Hu, Yanxin" uniqKey="Hu Y" first="Yanxin" last="Hu">Yanxin Hu</name><name sortKey="Jin, Yi" sort="Jin, Yi" uniqKey="Jin Y" first="Yi" last="Jin">Yi Jin</name><name sortKey="Sun, Lun Uan" sort="Sun, Lun Uan" uniqKey="Sun L" first="Lun-Quan" last="Sun">Lun-Quan Sun</name><name sortKey="Sun, Lun Uan" sort="Sun, Lun Uan" uniqKey="Sun L" first="Lun-Quan" last="Sun">Lun-Quan Sun</name><name sortKey="Wang, Ming" sort="Wang, Ming" uniqKey="Wang M" first="Ming" last="Wang">Ming Wang</name><name sortKey="Wang, Ming" sort="Wang, Ming" uniqKey="Wang M" first="Ming" last="Wang">Ming Wang</name><name sortKey="Wang, Ming" sort="Wang, Ming" uniqKey="Wang M" first="Ming" last="Wang">Ming Wang</name><name sortKey="Zhang, Guozhong" sort="Zhang, Guozhong" uniqKey="Zhang G" first="Guozhong" last="Zhang">Guozhong Zhang</name></country><country name="Canada"><noRegion><name sortKey="Wong, Jonathan" sort="Wong, Jonathan" uniqKey="Wong J" first="Jonathan" last="Wong">Jonathan Wong</name></noRegion></country><country name="Australie"><noRegion><name sortKey="Sun, Lun Uan" sort="Sun, Lun Uan" uniqKey="Sun L" first="Lun-Quan" last="Sun">Lun-Quan Sun</name></noRegion><name sortKey="Sun, Lun Uan" sort="Sun, Lun Uan" uniqKey="Sun L" first="Lun-Quan" last="Sun">Lun-Quan Sun</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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